Mary Gates Research Scholar, Winter 2024
Research Project: Optimization of Optogenetic Estradiol Sensor via Site-Directed Mutagenesis
Project Description: I am developing a genetically encoded fluorescent biosensor to measure estradiol, a hormone involved in reproductive health, bone density, cardiovascular function, and cognition. The sensor design uses the conformational change of the estrogen receptor’s ligand binding domain to alter the fluorescence output of the attached fluorescent protein, allowing real-time monitoring of hormone dynamics in living cells. The goal of my project is to optimize the sensor’s sensitivity and dynamic range, which will make it more reliable for studying how estradiol signaling contributes to neurodegenerative diseases like Alzheimer’s.
What have you learned throughout your research project?
I have learned how to think critically about experimental design and how small protein engineering changes can significantly improve biosensor performance. I’ve also gained hands-on experience with cloning, mutagenesis, and cell imaging, which has built both my technical skills and my confidence as a researcher.
What piece of advice do you have for future applicants?
When you’re working on your application, emphasize explaining why your project matters to you and what you’ve learned from it so far. Sharing your genuine interest and goals helps the reviewers understand your perspective. I also found it helpful to ask mentors or friends for feedback, since they can offer valuable insights and suggestions for your writing.